The extraction and analysis of benzodiazepines in tissues by enzymic digestion and high-performance liquid chromatography.

The extraction and analysis of benzodiazepines in tissues by enzymic digestion and high‐performance liquid chromatography

Authors: OSSELTON, M.D., HAMMOND, M.D., TWITCHETT, P.J.

Journal: Journal of Pharmacy and Pharmacology

Publication Date: 01/01/1977

Volume: 29

Issue: 1

Pages: 460-462

eISSN: 2042-7158

ISSN: 0022-3573

DOI: 10.1111/j.2042-7158.1977.tb11369.x

Abstract:

A simple, rapid and sensitive method is described for the analysis of unchanged benzodiazepine drugs and their metabolites, in human and animal tissues, using the proteolytic enzyme subtilisin Carlsberg followed by reverse‐phase liquid chromatography. The enzymic digestion yields far higher recoveries than conventional extraction methods, while the liquid chromatographic analysis allows the rapid separation and detection of nanogram quantities of these drugs without the need for elaborate “clean‐up” procedures. 1977 Royal Pharmaceutical Society of Great Britain

Source: Scopus

Authors: Osselton, M.D., Hammond, M.D., Twitchett, P.J.

Journal: J Pharm Pharmacol

Publication Date: 08/1977

Volume: 29

Issue: 8

Pages: 460-462

ISSN: 0022-3573

DOI: 10.1111/j.2042-7158.1977.tb11369.x

Abstract:

A simple, rapid and sensitive method is described for the analysis of unchanged benzodiazepine drugs and their metabolites, in human and animal tissues, using the proteolytic enzyme subtilisin Carlsberg followed by reverse-phase liquid chromatography. The enzymic digestion yields far higher recoveries than conventional extraction methods, while the liquid chromatogrpahic analysis allows the rapid separation and detection of nanogram quantities of these drugs without the need for elaborate "clean-up" procedures.

Source: PubMed

Preferred by: David Osselton