Comparison of Circulating Tumor DNA Assays for Molecular Residual Disease Detection in Early-Stage Triple-Negative Breast Cancer
Authors: Coakley, M., Villacampa, G., Sritharan, P., Swift, C., Dunne, K., Kilburn, L., Goddard, K., Pipinikas, C., Rojas, P., Emmett, W., Hall, P., Harper-Wynne, C., Hickish, T., Macpherson, I., Okines, A., Wardley, A., Wheatley, D., Waters, S., Palmieri, C., Winter, M., Cutts, R.J., Garcia-Murillas, I., Bliss, J., Turner, N.C.
Journal: Clinical Cancer Research
Publication Date: 15/02/2024
Volume: 30
Issue: 4
Pages: 895-903
eISSN: 1557-3265
ISSN: 1078-0432
DOI: 10.1158/1078-0432.CCR-23-2326
Abstract:Purpose: Detection of circulating tumor DNA (ctDNA) in patients who have completed treatment for early-stage breast cancer is associated with a high risk of relapse, yet the optimal assay for ctDNA detection is unknown. Experimental Design: The cTRAK-TN clinical trial prospectively used tumor-informed digital PCR (dPCR) assays for ctDNA molecular residual disease (MRD) detection in early-stage triple-negative breast cancer. We compared tumor-informed dPCR assays with tumor-informed personalized multimutation sequencing assays in 141 patients from cTRAK-TN. Results: MRD was first detected by personalized sequencing in 47.9% of patients, 0% first detected by dPCR, and 52.1% with both assays simultaneously (P < 0.001; Fisher exact test). The median lead time from ctDNA detection to relapse was 6.1 months with personalized sequencing and 3.9monthswith dPCR(P=0.004, mixed-effects Cox model). Detection of MRD at the first time point was associated with a shorter time to relapse compared with detection at subsequent time points (median lead time 4.2 vs. 7.1 months; P = 0.02). Conclusions: Personalized multimutation sequencing assays have potential clinically important improvements in clinical outcome in the early detection of MRD.
Source: Scopus
Comparison of Circulating Tumor DNA Assays for Molecular Residual Disease Detection in Early-Stage Triple-Negative Breast Cancer.
Authors: Coakley, M., Villacampa, G., Sritharan, P., Swift, C., Dunne, K., Kilburn, L., Goddard, K., Pipinikas, C., Rojas, P., Emmett, W., Hall, P., Harper-Wynne, C., Hickish, T., Macpherson, I., Okines, A., Wardley, A., Wheatley, D., Waters, S., Palmieri, C., Winter, M., Cutts, R.J., Garcia-Murillas, I., Bliss, J., Turner, N.C.
Journal: Clin Cancer Res
Publication Date: 16/02/2024
Volume: 30
Issue: 4
Pages: 895-903
eISSN: 1557-3265
DOI: 10.1158/1078-0432.CCR-23-2326
Abstract:PURPOSE: Detection of circulating tumor DNA (ctDNA) in patients who have completed treatment for early-stage breast cancer is associated with a high risk of relapse, yet the optimal assay for ctDNA detection is unknown. EXPERIMENTAL DESIGN: The cTRAK-TN clinical trial prospectively used tumor-informed digital PCR (dPCR) assays for ctDNA molecular residual disease (MRD) detection in early-stage triple-negative breast cancer. We compared tumor-informed dPCR assays with tumor-informed personalized multimutation sequencing assays in 141 patients from cTRAK-TN. RESULTS: MRD was first detected by personalized sequencing in 47.9% of patients, 0% first detected by dPCR, and 52.1% with both assays simultaneously (P < 0.001; Fisher exact test). The median lead time from ctDNA detection to relapse was 6.1 months with personalized sequencing and 3.9 months with dPCR (P = 0.004, mixed-effects Cox model). Detection of MRD at the first time point was associated with a shorter time to relapse compared with detection at subsequent time points (median lead time 4.2 vs. 7.1 months; P = 0.02). CONCLUSIONS: Personalized multimutation sequencing assays have potential clinically important improvements in clinical outcome in the early detection of MRD.
Source: PubMed
Comparison of Circulating Tumor DNA Assays for Molecular Residual Disease Detection in Early-Stage Triple-Negative Breast Cancer
Authors: Coakley, M., Villacampa, G., Sritharan, P., Swift, C., Dunne, K., Kilburn, L., Goddard, K., Pipinikas, C., Rojas, P., Emmett, W., Hall, P., Harper-Wynne, C., Hickish, T., Macpherson, I., Okines, A., Wardley, A., Wheatley, D., Waters, S., Palmieri, C., Winter, M., Cutts, R.J., Garcia-Murillas, I., Bliss, J., Turner, N.C.
Journal: CLINICAL CANCER RESEARCH
Publication Date: 16/02/2024
Volume: 30
Issue: 4
Pages: 895-903
eISSN: 1557-3265
ISSN: 1078-0432
DOI: 10.1158/1078-0432.CCR-23-2326
Source: Web of Science
Comparison of Circulating Tumor DNA Assays for Molecular Residual Disease Detection in Early-Stage Triple-Negative Breast Cancer
Authors: Coakley, M., Villacampa, G., Sritharan, P., Swift, C., Dunne, K., Kilburn, L., Goddard, K., Pipinikas, C., Rojas, P., Emmett, W., Hall, P., Harper-Wynne, C., Hickish, T., Macpherson, I., Okines, A., Wardley, A., Wheatley, D., Waters, S., Palmieri, C., Winter, M., Cutts, R.J., Garcia-Murillas, I., Bliss, J., Turner, N.C.
Journal: CLINICAL CANCER RESEARCH
Publication Date: 2024
Volume: 30
Issue: 4
Pages: 895-903
eISSN: 1557-3265
ISSN: 1078-0432
DOI: 10.1158/1078-0432.CCR-23-2326
Source: Web of Science
Comparison of Circulating Tumor DNA Assays for Molecular Residual Disease Detection in Early-Stage Triple-Negative Breast Cancer
Authors: Coakley, M., Villacampa, G., Sritharan, P., Swift, C., Dunne, K., Kilburn, L., Goddard, K., Pipinikas, C., Rojas, P., Emmett, W., Hall, P., Harper-Wynne, C., Hickish, T., Macpherson, I., Okines, A., Wardley, A., Wheatley, D., Waters, S., Palmieri, C., Winter, M., Cutts, R.J., Garcia-Murillas, I., Bliss, J., Turner, N.C.
Journal: CLINICAL CANCER RESEARCH
Publication Date: 2024
Volume: 30
Issue: 4
Pages: 895-903
eISSN: 1557-3265
ISSN: 1078-0432
DOI: 10.1158/1078-0432.CCR-23-2326
Source: Web of Science
Comparison of Circulating Tumor DNA Assays for Molecular Residual Disease Detection in Early-Stage Triple-Negative Breast Cancer
Authors: Coakley, M., Villacampa, G., Sritharan, P., Swift, C., Dunne, K., Kilburn, L., Goddard, K., Pipinikas, C., Rojas, P., Emmett, W., Hall, P., Harper-Wynne, C., Hickish, T., Macpherson, I., Okines, A., Wardley, A., Wheatley, D., Waters, S., Palmieri, C., Winter, M., Cutts, R.J., Garcia-Murillas, I., Bliss, J., Turner, N.C.
Journal: CLINICAL CANCER RESEARCH
Publication Date: 2024
Volume: 30
Issue: 4
Pages: 895-903
eISSN: 1557-3265
ISSN: 1078-0432
DOI: 10.1158/1078-0432.CCR-23-2326
Source: Web of Science
Comparison of Circulating Tumor DNA Assays for Molecular Residual Disease Detection in Early-Stage Triple-Negative Breast Cancer.
Authors: Coakley, M., Villacampa, G., Sritharan, P., Swift, C., Dunne, K., Kilburn, L., Goddard, K., Pipinikas, C., Rojas, P., Emmett, W., Hall, P., Harper-Wynne, C., Hickish, T., Macpherson, I., Okines, A., Wardley, A., Wheatley, D., Waters, S., Palmieri, C., Winter, M., Cutts, R.J., Garcia-Murillas, I., Bliss, J., Turner, N.C.
Journal: Clinical cancer research : an official journal of the American Association for Cancer Research
Publication Date: 02/2024
Volume: 30
Issue: 4
Pages: 895-903
eISSN: 1557-3265
ISSN: 1078-0432
DOI: 10.1158/1078-0432.ccr-23-2326
Abstract:Purpose
Detection of circulating tumor DNA (ctDNA) in patients who have completed treatment for early-stage breast cancer is associated with a high risk of relapse, yet the optimal assay for ctDNA detection is unknown.Experimental design
The cTRAK-TN clinical trial prospectively used tumor-informed digital PCR (dPCR) assays for ctDNA molecular residual disease (MRD) detection in early-stage triple-negative breast cancer. We compared tumor-informed dPCR assays with tumor-informed personalized multimutation sequencing assays in 141 patients from cTRAK-TN.Results
MRD was first detected by personalized sequencing in 47.9% of patients, 0% first detected by dPCR, and 52.1% with both assays simultaneously (P < 0.001; Fisher exact test). The median lead time from ctDNA detection to relapse was 6.1 months with personalized sequencing and 3.9 months with dPCR (P = 0.004, mixed-effects Cox model). Detection of MRD at the first time point was associated with a shorter time to relapse compared with detection at subsequent time points (median lead time 4.2 vs. 7.1 months; P = 0.02).Conclusions
Personalized multimutation sequencing assays have potential clinically important improvements in clinical outcome in the early detection of MRD.Source: Europe PubMed Central